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The second reaction cycle in NO synthesis involves O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to generate ferric enzyme, NO, citrulline, and water as products (Fig. 9) - circular dichroism. As drawn in my explanation Fig - https://calendly.com/olisclarity1/30min. 9, the reaction might also include electron transfer from H4B to the Fe, IIO2 intermediate
l9 As revealed in Fig. 9, this could be because the H4B radical serve as an electron sink in a later point in the response, so as to permit NOS to, Fig. 9. A design for NORA oxidation by NOSoxy in the single turnover reaction. Oxygen binds to the ferrous protein to form the Fe, IIO2 types, which upon receiving an electron from H4B forms a putative hemeperoxo intermediate that reacts with NOHA.